Journal article
Altered gp130 signalling ameliorates experimental colitis via myeloid cell-specific STAT3 activation and myeloid-derived suppressor cells
J Däbritz, LM Judd, HV Chalinor, TR Menheniott, AS Giraud
Scientific Reports | Published : 2016
DOI: 10.1038/srep20584
Abstract
STAT3 regulates the expansion of myeloid-derived suppressor cells (MDSCs) during inflammation, infection and cancer. Hyperactivation of STAT3 in gp130 757F/F mice is associated with protection from experimental colitis. This study determined mechanisms for this protection and compared this to mice with myeloid-specific STAT3-deficiency (LysMcre/STAT3 flox; gp130 757F/F LysMcre/STAT3 flox). Acute and chronic colitis was induced and colons were removed for histological, mRNA and protein analysis. Cell populations from spleen, mesenteric lymph node and colon were analyzed for different myeloid cell populations using flow cytometry. Functions of MDSCs and LPS-stimulated peritoneal macrophages we..
View full abstractGrants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was supported by a research fellowship (J.D.) from the German Research Foundation (DFG DA1161/5-1) and a career development award (J.D.) from the German Society of Gastroenterology, Digestive and Metabolic Diseases (DGVS). This work was also supported by a project grant (#509165), career development awards (L.M.J., T.R.M.) and a senior research fellowship (A.S.G.) from the National Health and Medical Research Council (NHMRC) of Australia. The authors thank the United European Gastroenterology for the National Scholar Award 2013 and also for a travel grant (#UEG13-ABS-1586), which supported the presentation of the research at the 21st United European Gastroenterology Week 2013 in Berlin, Germany.